Dodecyltrimethylammonium bromide (DTAB) is a versatile quaternary ammonium surfactant that has numerous applications in cell biology and biochemical research. Its distinct qualities make it an effective tool for solubilizing proteins and peptides, extracting DNA, and lysing cells. In proteomics research, DTAB is essential for protein solubilization and purification. Its ability to disrupt hydrophobic bonds and electrostatic forces enables it to effectively solubilize membrane-bound proteins and peptides, making them available for investigation and characterisation. This characteristic is especially relevant in proteomics investigations that involve membrane proteins, which are notoriously difficult to solubilize using conventional methods.