Tth DNA Ligase

NAD-dependent recombinant ligase from Thermus thermophilus. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them. Therefore a single-base substitution can be detected. High thermostability allows ligation using high-stringency hybridization conditions. High specificity and stringency permits sensitive detection of SNPs. Equivalent of Ampligase® (Epicentre).